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CD130/gp130 Kit ELISA
IL6ST
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4885740
Détail du produit CD130/gp130 Kit ELISA
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Antigène
Voir toutes CD130/gp130 (IL6ST) Kits ELISA
CD130/gp130 (IL6ST)
(Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
Reactivité
Toutes les réactivités sur CD130/gp130 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Mouse gp130 (sgp130) SpeedELISA Kit.
Marque
SpeedELISA
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Attributs du produit
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Durée du test
3 h
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CD130/gp130 (IL6ST) Kits ELISA
CD130/gp130 (IL6ST)
(Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
Autre désignation
Gp130 (IL6ST Produits )
Synonymes
CD130 Kit ELISA, CDW130 Kit ELISA, GP130 Kit ELISA, IL-6RB Kit ELISA, 5133400A03Rik Kit ELISA, AA389424 Kit ELISA, BB405851 Kit ELISA, D13Ertd699e Kit ELISA, gp130 Kit ELISA, Ac1055 Kit ELISA, Gp130 Kit ELISA, Il-6rb Kit ELISA, IL-6 Kit ELISA, interleukin 6 signal transducer Kit ELISA, IL6ST Kit ELISA, Il6st Kit ELISA, il6st Kit ELISA
ID gène
16195
UniProt
Q00560
Pathways
Signalistation JAK/STAT , Cellular Glucan Metabolic Process , Autophagy , Smooth Muscle Cell Migration , Cancer Immune Checkpoints
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