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PAK2 Kit ELISA

PAK2 Reactivité: Humain pSer20 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886234
  • Antigène Voir toutes PAK2 Kits ELISA
    PAK2 (P21-Activated Kinase 2 (PAK2))
    Épitope
    pSer20, total
    Reactivité
    • 7
    • 4
    • 4
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-PAK2 (Ser20) and Total PAK2 ELISA Kit. This assay semi-quantitatively measures phophorylated PAK2 (Ser20) and Total PAK2 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human PAK2 phosphorylated at site Serine-20 as well as total PAK2.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product PAK2 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes PAK2 Kits ELISA
    PAK2 (P21-Activated Kinase 2 (PAK2))
    Autre désignation
    PAK2 (PAK2 Produits)
    Synonymes
    PAK65 Kit ELISA, PAKgamma Kit ELISA, gamma-PAK Kit ELISA, 5330420P17Rik Kit ELISA, A130002K10Rik Kit ELISA, AI836325 Kit ELISA, D16Ertd269e Kit ELISA, PAK-2 Kit ELISA, mKIAA4182 Kit ELISA, pak2 Kit ELISA, cb422 Kit ELISA, kinase Kit ELISA, zgc:91798 Kit ELISA, wu:fb71h01 Kit ELISA, si:dz198m22.1 Kit ELISA, pak65 Kit ELISA, pakgamma Kit ELISA, PAKI Kit ELISA, Pak3 Kit ELISA, CH73-159L23.1 Kit ELISA, wu:fc52b03 Kit ELISA, zgc:113857 Kit ELISA, p21 (RAC1) activated kinase 2 Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2 Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2a Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2b Kit ELISA, PAK2 Kit ELISA, Pak2 Kit ELISA, pak2a Kit ELISA, pak2.S Kit ELISA, pak2b Kit ELISA
    ID gène
    5062
    UniProt
    Q13177
    Pathways
    Signalisation MAPK, Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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