PAK2 Kit ELISA

N° du produit ABIN5526726

Détail du produit PAK2 Kit ELISA

Antigène: PAK2 (P21-Activated Kinase 2 (PAK2))

Épitope: pSer20, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Cell-Based ELISA Kit. This assay semi-quantitatively measures PAK2 phosphorylated at Serine-20 as well as total PAK2 in adherent cell lines.

Type d'échantillon: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human PAK2 phosphorylated at site Serine-20 as well as total PAK2.

Attributs du produit:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Ingrédients:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: PAK2 (P21-Activated Kinase 2 (PAK2))

Autre désignation: PAK2 (PAK2 Produits)

Synonymes: PAK65 Kit ELISA, PAKgamma Kit ELISA, gamma-PAK Kit ELISA, 5330420P17Rik Kit ELISA, A130002K10Rik Kit ELISA, AI836325 Kit ELISA, D16Ertd269e Kit ELISA, PAK-2 Kit ELISA, mKIAA4182 Kit ELISA, pak2 Kit ELISA, cb422 Kit ELISA, kinase Kit ELISA, zgc:91798 Kit ELISA, wu:fb71h01 Kit ELISA, si:dz198m22.1 Kit ELISA, pak65 Kit ELISA, pakgamma Kit ELISA, PAKI Kit ELISA, Pak3 Kit ELISA, CH73-159L23.1 Kit ELISA, wu:fc52b03 Kit ELISA, zgc:113857 Kit ELISA, p21 (RAC1) activated kinase 2 Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2 Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2a Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog Kit ELISA, p21 protein (Cdc42/Rac)-activated kinase 2b Kit ELISA, PAK2 Kit ELISA, Pak2 Kit ELISA, pak2a Kit ELISA, pak2.S Kit ELISA, pak2b Kit ELISA

ID gène: 5062

UniProt: Q13177

Pathways: Signalisation MAPK, Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha