Rituximab Kit ELISA

N° du produit ABIN4886396

Kit ELISA Rituximab Humain, Souris et Rat, Colorimetric test pour la quantification de Humain, Souris et Rat Rituximab.

Aperçu rapide pour Rituximab Kit ELISA (ABIN4886396)

Antigène: Rituximab

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 5 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Rituximab Kit ELISA

Seuil minimal de détection: 5 ng/mL

Fonction: Quantification of Rituximab in biological matrices

Analytical Method: Quantitative

Specificité: Rituximab (Rituxan)

Réactivité croisée (Details): hIgG1 and Infliximab prepared at 250 ng/ mL were assayed and exhibited no cross-reactivity or interference

Ingrédients:

• Coated microtiter plate, 96 wells
• Calibrator diluent. - 1.8ml
• Calibrator 12ul 10X wash buffer - 25ml
• Assay buffer - 50ml
• 1000X detection reagent - 17ul TMB - 12ml
• TMB stop solution - 12ml
• Plate sealers - 3

Matériel non inclus:

• Precision pipettes calibrated to deliver 5-1000μL
• Multi-channel pipette calibrated to deliver 50-200μL
• Plate shaker
• Disposable tips
• Vortex-Mixe
• Distilled or de-ionized water
• Microplate reader capable of reading 450nm with background subtraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 15 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: The Rituximab ELISA kit is designed to measure free Rituximab with high specificity and sensitivity . This assay employs the sandwich enzyme immunoassay technique. A precoated anti-Rituximab 96 well plate is provided. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Rituximab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Rituximab present in test samples and the concentration is calculated from the standard series.

Préparation des réactifs:

Prepare the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1/10 before use (for example add 20 mL concentrate to 180 mL ultra-pure water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11 μL concentrate to 11 mL of assay buffer). Mix well. 3. Preparation of Calibrators: Prepare calibrators with concentrations ranging from 5000 ng/mL to 250 ng/ mL. The following is an example calibrator curve.

Prélèvement de l'échantillon: This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20 °C for up to 1 year.

Préparation de l'échantillon:

Dilute calibrators and test samples 1/50 with assay buffer (for example add 5μL of prepared calibrator or sample to 245μL of assay buffer). Mix well. *Note that test samples may require further dilution when peak values are predicted to exceed 5 μg/mL. Do not store diluted samples.

Procédure de l'essai:

This assay employs the sandwich enzyme immunoassay technique. Anti- Rituximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Rituximab present in biological matrices is bound by the immobilized anti- Rituximab antibody. After washing away any unbound substances, enzyme linked anti- Rituximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Rituxan® (domain residing in Fc portion of the Rituxan® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Rituximab present in test samples. The color development is stopped and the intensity of the color is measured.

Calcul des résultats:

1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.

Précision du teste: Intra-assay precision: <10%
Inter-assay precision: <10%

Restrictions: For Research Use only

Stockage

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: -20C, 1 year

Date de péremption: 12 months

Détail du antigène

Antigène: Rituximab

Classe de substances: Antibody

Sujet: Rituximab is a chimeric monoclonal antibody used in the treatment of diseases characterized by abnormal or excessive B cells. Rituximab targets CD20, which is expressed on the surface of B cells, to induce apoptosis in B cells and is used in the treatment of leukemias and lymphomas, some autoimmune disorders, and organ transplant. A quantitative method for Rituximab has been developed and optimized for pharmacokinetic assessment of samples.

ID gène: 931