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FYN Kit ELISA

FYN Reactivité: Humain pTyr530 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN4889771
  • Antigène Voir toutes FYN Kits ELISA
    FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
    Épitope
    pTyr530
    Reactivité
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-FYN (Tyr530) ELISA Kit. This assay semi-quantitatively measures FYN phosphorylated at Tyrosine-530 in cell lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human FYN phosphorylated at site Tyrosine-530.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling FYN Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes FYN Kits ELISA
    FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
    Autre désignation
    FYN (FYN Produits)
    Synonymes
    im:6908851 Kit ELISA, si:dkey-119m7.5 Kit ELISA, zgc:109996 Kit ELISA, c-fyn Kit ELISA, cfyn-a Kit ELISA, SLK Kit ELISA, SYN Kit ELISA, p59-FYN Kit ELISA, PKC Kit ELISA, fyn Kit ELISA, zgc:86720 Kit ELISA, AI448320 Kit ELISA, AW552119 Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase S homeolog Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase b Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase L homeolog Kit ELISA, FYN proto-oncogene, Src family tyrosine kinase a Kit ELISA, Fyn proto-oncogene Kit ELISA, FYN Kit ELISA, fyn.S Kit ELISA, fynb Kit ELISA, fyn.L Kit ELISA, Fyn Kit ELISA, fyna Kit ELISA
    ID gène
    2534
    UniProt
    P06241
    Pathways
    Signalistation JAK/STAT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
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