TNF alpha Kit ELISA

N° du produit ABIN4987126

Kit ELISA Porc TNF alpha, test Colorimetric pour la quantification de Porc TNF alpha.

Aperçu rapide pour TNF alpha Kit ELISA (ABIN4987126)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.03-2.0 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Détail du produit TNF alpha Kit ELISA

Seuil minimal de détection: 0.03 ng/mL

Analytical Method: Quantitative

Specificité: Natural and recombinant Swine TNF-α Ligand

Sensibilité: 15 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha

Sujet: Tumor necrosis factor alpha (TNF- ), also known as cachectin, is a member of the TNF ligand superfamily and has been designated TNFSF1A. It binds to the same cell surface receptors, and shares some biological functions with TNF- /TNFSF1B. TNF- ?inhibits the growth of certain tumors. It also plays a critical role in normal host resistance to infection, serving as an immunomodulator and as a mediator of inflammatory responses. Over-production of TNF has been implicated in a number of pathological conditions, including cachexia, septic shock, and autoimmune disorders (1 - 4). TNF- ?is produced primarily by activated macrophages (1 - 6). Various other porcine cell types, including NK cells (6), keratinocytes (7), vascular smooth muscle cells (8), and granulosa lutein cells (9) are also known to produce TNF- . The porcine TNF- ?gene product is a 232 amino acid (aa) residue type II membrane glycoprotein containing a 35 aa cytoplasmic domain, a 21 aa transmembrane domain and a 178 aa extracellular domain (10 - 13). The 156 aa residue soluble TNF- ?is released from the C-terminus of the membrane protein by TNF- ?converting enzyme (TACE, ADAM17), a member of the ADAM (a disintegrin and metalloprotease domain) family of metalloproteases(10, 11, 14). The biologically active TNF- ?has been shown to exist as a trimer (1 - 4). Porcine TNF- ?is active on mouse cells and shares 89 % and 79 % aa sequence identity with human and mouse TNF- , respectively (10, 14).Two distinct TNF receptors, referred to as type I (type B, p55, or TNFRSF1A) and type II (type A, p75, or TNFRSF1B), that specifically bind TNF- ?and TNF- ?with equal affinities are known (15 - 17). The two TNF receptors share aa sequence homology in their extracellular but not their cytoplasmic domains, suggesting that the two receptors employ different signal transduction pathways. Soluble forms of both types of receptors have been found in human and mouse serum (18 - 20). These soluble receptors are capable of neutralizing the biological activities of the TNFs and may serve to modulate the activities of TNF.? Porcine TNF RI shares 79 % and 72 % aa homology with the human and mouse TNF RI, respectively (21 - 23).

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome