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15d-PGJ2 Kit ELISA

15d-PGJ2 Reactivité: Différentes espèces Colorimetric Competition ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum
N° du produit ABIN4993245
  • Antigène Tous les produits 15d-PGJ2
    15d-PGJ2 (15-Deoxy-Prostaglandin J2 (15d-PGJ2))
    Reactivité
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    Différentes espèces
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    3.13 ng/mL - 200 ng/mL
    Seuil minimal de détection
    3.13 ng/mL
    Application
    ELISA
    Fonction
    The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of 15d-PGJ2 in serum, plasma and other biological fluids
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This kit recognizes natural and recombinant 15d-PGJ2. No signif icant cross-reactivity or interference between 15d-PGJ2 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between 15d-PGJ2 and all the analogues.
    Sensibilité
    1.88 ng/mL
    Ingrédients
    Micro ELISA Plate(Dismountable)
    Reference Standard
    Concentrated Biotinylated Detection Ab (100×)
    Concentrated HRP Conjugate (100×)
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solution
    Plate Sealer
    Product Description
    Certificate of Analysis
    Matériel non inclus
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
  • Commentaires

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Volume d'échantillon
    50 µL
    Plaque
    Pre-coated
    Protocole
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Antigène Tous les produits 15d-PGJ2
    15d-PGJ2 (15-Deoxy-Prostaglandin J2 (15d-PGJ2))
    Autre désignation
    15-Deoxy-Delta12,14-prostaglandin J2 (15d-PGJ2) (15d-PGJ2 Produits)
    Classe de substances
    Hormone
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