MYLK Kit ELISA
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- Antigène Voir toutes MYLK Kits ELISA
- MYLK (Myosin Light Chain Kinase (MYLK))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 23.438-1500 pg/mL
- Seuil minimal de détection
- 23.438 pg/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MLCK/MYLK in Mouse serum, plasma and other biological fluids
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This kit recognizes natural and recombinantMouseMLCK/MYLK. No significant cross-reactivity or interference between MouseMLCK/MYLK and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseMLCK/MYLK and all the analogues.
- Sensibilité
- 14.063 pg/mL
- Ingrédients
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Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis - Matériel non inclus
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Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer - Featured
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- Commentaires
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Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - Volume d'échantillon
- 100 µL
- Plaque
- Pre-coated
- Protocole
- This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MLCK/MYLK. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MLCK/MYLKand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MLCK/MYLK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MLCK/MYLK.You can calculate the concentration of MLCK/MYLKin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- Restrictions
- For Research Use only
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- Conseil sur la manipulation
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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- Antigène Voir toutes MYLK Kits ELISA
- MYLK (Myosin Light Chain Kinase (MYLK))
- Autre désignation
- Myosin light chain kinase (MLCK/MYLK) (MYLK Produits)
- Synonymes
- AAT7 Kit ELISA, KRP Kit ELISA, MLCK Kit ELISA, MLCK1 Kit ELISA, MLCK108 Kit ELISA, MLCK210 Kit ELISA, MSTP083 Kit ELISA, MYLK1 Kit ELISA, smMLCK Kit ELISA, 9530072E15Rik Kit ELISA, A930019C19Rik Kit ELISA, AW489456 Kit ELISA, Mlck Kit ELISA, SMMLCK Kit ELISA, mlck Kit ELISA, fj60a06 Kit ELISA, mlck1a Kit ELISA, mylk Kit ELISA, wu:fj60a06 Kit ELISA, zmlck1 Kit ELISA, myosin light chain kinase Kit ELISA, myosin, light polypeptide kinase Kit ELISA, myosin, light chain kinase a Kit ELISA, MYLK Kit ELISA, Mylk Kit ELISA, mylka Kit ELISA
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