EGF Kit ELISA

N° du produit ABIN5026920

Kit ELISA EGF Humain, Colorimetric test pour la quantification de Humain EGF.

Aperçu rapide pour EGF Kit ELISA (ABIN5026920)

Antigène: EGF (Epidermal Growth Factor (EGF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.9-250 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit EGF Kit ELISA

Seuil minimal de détection: 3.9 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 2.34 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human EGF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: <2.34 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human EGF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human EGF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EGF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human EGF can be calculated using the standard curve.  

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF (Epidermal Growth Factor)

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation