CYP3A4 Kit ELISA

N° du produit ABIN5067926

Détail du produit CYP3A4 Kit ELISA

Antigène: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The CYP3A4 (Cytochrome P450 3A4) ELISA Kit (Human) is a quantitative sandwich assay for the detection of CYP3A4 in human serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: > 46.9 pg/mL

Attributs du produit: CYP3A4 (Cytochrome P450 3A4) ELISA Kit (Human)

Ingrédients:

• *355152A: Microtiter Strips, 8x12 wells.
• *355152B: Standard, 2 vials
• 355152C: Sample/Standard Dilution Buffer, 1x20ml
• 355152D: Antibody-Biotin (Concentrated), 1x120ul
• 355152E: Antibody Dilution Buffer, 1x10ml
• 355152F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 355152G: SABC Dilution Buffer, 1x10ml
• 355152H: TMB Substrate, 1x10ml
• 355152J: Stop Solution, 1x10ml
• 355152K: Wash Buffer (25X), 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP3A4. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CYP3A4. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP3A4, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CYP3A4 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Autre désignation: CYP3A4 (Cytochrome P450 3A4) (CYP3A4 Produits)

Synonymes: CP33 Kit ELISA, CP34 Kit ELISA, CYP3A Kit ELISA, CYP3A3 Kit ELISA, CYPIIIA3 Kit ELISA, CYPIIIA4 Kit ELISA, HLP Kit ELISA, NF-25 Kit ELISA, P450C3 Kit ELISA, P450PCN1 Kit ELISA, MGC108372 Kit ELISA, CYP3A80 Kit ELISA, CYP3A4 Kit ELISA, CYP3A21 Kit ELISA, CYPIIIA21 Kit ELISA, CYP3A12 Kit ELISA, cytochrome P450 family 3 subfamily A member 4 Kit ELISA, cytochrome P450 family 3 subfamily A member 43 Kit ELISA, cytochrome P450 3A4 Kit ELISA, cytochrome P450, subfamily IIIA, polypeptide 4 Kit ELISA, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 Kit ELISA, cytochrome P450, family 3, subfamily A, polypeptide 4 Kit ELISA, CYP3A4 Kit ELISA, CYP3A43 Kit ELISA, PTRG_01782 Kit ELISA, PTRG_06060 Kit ELISA, cyp3a4 Kit ELISA

Pathways: Steroid Hormone Biosynthesis