PDGF-BB Homodimer Kit ELISA

N° du produit ABIN5067997

Détail du produit

Antigène: PDGF-BB Homodimer

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The Mouse Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit is a quantitative sandwich assay for the detection of PDGF-BB in mouse serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 23.438 pg/mL

Attributs du produit: PDGF-BB (Platelet-Derived Growth Factor-BB) ELISA Kit (Mouse)

Ingrédients:

• *357966A: Microtiter Strips, 8x12 wells.
• *357966B: Standard, 2 vials
• 357966C: Sample/Standard Dilution Buffer, 1x20ml
• 357966D: Antibody-Biotin (Concentrated), 1x120ul
• 357966E: Antibody Dilution Buffer, 1x10ml
• 357966F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 357966G: SABC Dilution Buffer, 1x10ml
• 357966H: TMB Substrate, 1x10ml
• 357966J: Stop Solution, 1x10ml
• 357966K: Wash Buffer (25X), 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PDGF-BB. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGF-BB, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-BB in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: PDGF-BB Homodimer

Autre désignation: PDGF-BB (Platelet-Derived Growth Factor-BB)

Synonymes: PDGF-2 Kit ELISA, PDGF2 Kit ELISA, SIS Kit ELISA, SSV Kit ELISA, c-sis Kit ELISA, PDGF-B Kit ELISA, Sis Kit ELISA, platelet derived growth factor subunit B Kit ELISA, platelet derived growth factor, B polypeptide Kit ELISA, PDGFB Kit ELISA, Pdgfb Kit ELISA