NEDD9 Kit ELISA

N° du produit ABIN5068005

Détail du produit NEDD9 Kit ELISA

Antigène: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The NEDD9 (Enhancer of filamentation 1) ELISA Kit (Human) is a quantitative sandwich assay for the detection of NEDD9 in human serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: > 0.094 ng/mL

Attributs du produit: NEDD9 (Enhancer of filamentation 1) ELISA Kit (Human)

Ingrédients:

• *357566A: Microtiter Strips, 8x12 wells.
• *357566B: Standard, 2 vials
• 357566C: Sample/Standard Dilution Buffer, 1x20ml
• 357566D: Antibody-Biotin (Concentrated), 1x120ul
• 357566E: Antibody Dilution Buffer, 1x10ml
• 357566F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 357566G: SABC Dilution Buffer, 1x10ml
• 357566H: TMB Substrate, 1x10ml
• 357566J: Stop Solution, 1x10ml
• 357566K: Wash Buffer (25X), 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEDD9. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to NEDD9. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEDD9, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEDD9 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Autre désignation: NEDD9 (Enhancer of filamentation 1) (NEDD9 Produits)

Synonymes: NEDD9 Kit ELISA, CAS-L Kit ELISA, CAS2 Kit ELISA, CASL Kit ELISA, CASS2 Kit ELISA, HEF1 Kit ELISA, Cas-L Kit ELISA, CasL Kit ELISA, E230025G09Rik Kit ELISA, neural precursor cell expressed, developmentally down-regulated 9 Kit ELISA, neural precursor cell expressed, developmentally down-regulated gene 9 Kit ELISA, NEDD9 Kit ELISA, Nedd9 Kit ELISA

UniProt: Q14511