TNF alpha Kit ELISA

N° du produit ABIN5522445

Kit ELISA TNF alpha Rat, Colorimetric test pour la quantification de Rat TNF alpha et a été mentionné dans 3+ publications.

Aperçu rapide pour TNF alpha Kit ELISA (ABIN5522445)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.906 pg/mL - 250 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit TNF alpha Kit ELISA

Seuil minimal de détection: 3.906 pg/mL

Fonction: Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with TNF-α , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Normal Rat serum (n=15) 1/2 dilution ND-25pg/mL Normal Rat plasma (EDTA, Citrate , heparin) (n=15) 1/2 dilution ND-30pg/mL Rat spleen cells were cultured with 5 %FBS + 1640 + double antibody for 48 hours 1/2dilution 15pg/mL Rat spleen cells were cultured with 5 %FBS + 1640 + double antibody + 100 ng/mL LPS for 48 hours 1/20 dilution 865pg/mL Rat spleen cells were cultured with 5 %FBS+ 1640 + double antibody +100 ng/mL LPS for 48 hours. After that, 300 ng/mL Brefeldin A (BFA) was added and cultured for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 2.1 mg/mL) to detect. 1/50 dilution 1.2 ng/mg(total protein) 2. KD sample validation (Detect TNF-α-KD NR8383 cells): Sample Type Dilution Ratio Content Wild NR8383 cells were stimulated with 1 μg/mL LPS for 12 hours to detect cell culture supernatant 1/2-1/10 dilution 635pg/mL NR8383 cells (+siRNA) were stimulated with 1 μg/mL LPS for 12 hours to detect cell culture supernatants 1/2 dilution ND NR8383 cells (+siRNA) were treated with 1 μg/mL LPS for 12 hours and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 1.28 mg/mL) to detect. 1/2 dilution 10pg/mg(total protein) Note:ND is lower than the sensitivity of the kit and was not detected 3. Antibody by WB KD validation (Detect TNF-α-KO NR8383 cells):

Sensibilité: 2.344 pg/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Volume d'échantillon: 100 μL

Durée du test: 4 h

Plaque: Pre-coated

Protocole: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Références pour TNF alpha Kit ELISA (ABIN5522445)

Li, Deng, Shang, Wang, Xiao: "Blocking TRPA1 and TNF-α Signal Improves Bortezomib-Induced Neuropathic Pain." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 51, Issue 5, pp. 2098-2110, (2019) (PubMed).

Ranneh, Akim, Hamid, Khazaai, Fadel, Mahmoud: "Stingless bee honey protects against lipopolysaccharide induced-chronic subclinical systemic inflammation and oxidative stress by modulating Nrf2, NF-κB and p38 MAPK." dans: Nutrition & metabolism, Vol. 16, pp. 15, (2019) (PubMed).

Bahtiar, Nurazizah, Roselina, Tambunan, Arsianti et al.: "Ethanolic extracts of babandotan leaves (Ageratum conyzoides L.) prevents inflammation and proteoglycan degradation by inhibiting TNF-α and MMP-9 on osteoarthritis rats induced by monosodium ..." dans: Asian Pacific journal of tropical medicine, Vol. 10, Issue 3, pp. 270-277, (2017) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha

Sujet: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

UniProt: P16599

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome