CAP1 Kit ELISA

N° du produit ABIN5651438

Détail du produit CAP1 Kit ELISA

Antigène: CAP1 (CAP, Adenylate Cyclase-Associated Protein 1 (CAP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adenylyl Cyclase Associated Protein 1 (CAP1). No significant cross-reactivity or interference between Adenylyl Cyclase Associated Protein 1 (CAP1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylyl Cyclase Associated Protein 1 (CAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylyl Cyclase Associated Protein 1 (CAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylyl Cyclase Associated Protein 1 (CAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylyl Cyclase Associated Protein 1 (CAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylyl Cyclase Associated Protein 1 (CAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylyl Cyclase Associated Protein 1 (CAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CAP1 (CAP, Adenylate Cyclase-Associated Protein 1 (CAP1))

Autre désignation: Adenylyl Cyclase Associated Protein 1 (CAP1 Produits)

Synonymes: CAP Kit ELISA, CAP1-PEN Kit ELISA, CAP1 Kit ELISA, DKFZp459E1319 Kit ELISA, cap1 Kit ELISA, Mch1 Kit ELISA, fj98f01 Kit ELISA, zgc:63872 Kit ELISA, wu:fj98f01 Kit ELISA, cyclase associated actin cytoskeleton regulatory protein 1 Kit ELISA, adenylyl cyclase-associated protein 1 Kit ELISA, Cap1 CAP, adenylate cyclase-associated protein 1 Kit ELISA, CAP, adenylate cyclase-associated protein 1 (yeast) Kit ELISA, adenylate cyclase associated protein 1 Kit ELISA, adenylyl cyclase-associated protein Cap1 Kit ELISA, CAP1 Kit ELISA, LOC5580331 Kit ELISA, cap1 Kit ELISA, Cap1 Kit ELISA

Sujet:

Gene Name: Adenylyl Cyclase Associated Protein 1

Gene Aliases: CAP, CAP1-PEN

ID gène: 10487

UniProt: Q01518

Pathways: cAMP Metabolic Process