ATP7B Kit ELISA

N° du produit ABIN5652087

Détail du produit ATP7B Kit ELISA

Antigène: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). No significant cross-reactivity or interference between ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Cu++ Transporting Beta Polypeptide (ATP7b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Autre désignation: ATPase, Cu++ Transporting Beta Polypeptide (ATP7B Produits)

Synonymes: PWD Kit ELISA, WC1 Kit ELISA, WD Kit ELISA, WND Kit ELISA, Atp7a Kit ELISA, tx Kit ELISA, Hts Kit ELISA, PINA Kit ELISA, Wd Kit ELISA, ATP7B Kit ELISA, ATPase copper transporting beta Kit ELISA, ATPase, Cu++ transporting, beta polypeptide Kit ELISA, ATPase, Cu++ transporting, beta polypeptide (Wilson disease) Kit ELISA, copper-transporting ATPase 2 Kit ELISA, ATP7B Kit ELISA, Atp7b Kit ELISA, atp7b Kit ELISA, LOC592143 Kit ELISA

Sujet:

Gene Name: ATPase, Cu++ Transporting Beta Polypeptide

Gene Aliases: PWD, WC1, WD, WND, Wilson Disease Protein, Copper pump 2, Wilson disease-associated protein

ID gène: 540

UniProt: P35670

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process