ATP5B Kit ELISA

N° du produit ABIN5652091

Détail du produit ATP5B Kit ELISA

Antigène: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). No significant cross-reactivity or interference between ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) and analogues was observed.

Sensibilité: 5.1 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Autre désignation: ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5B Produits)

Synonymes: atpmb Kit ELISA, atpsb Kit ELISA, Atpsyn-beta Kit ELISA, hm:zehn0534 Kit ELISA, im:6793121 Kit ELISA, wu:fj38d01 Kit ELISA, zgc:111961 Kit ELISA, ATPMB Kit ELISA, ATPSB Kit ELISA, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide Kit ELISA, ATP synthase subunit beta, mitochondrial Kit ELISA, ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit Kit ELISA, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide S homeolog Kit ELISA, ATP5B Kit ELISA, atp5b Kit ELISA, Atp5b Kit ELISA, LOC100401662 Kit ELISA, LOC100635763 Kit ELISA, atp5b.S Kit ELISA

Sujet:

Gene Name: ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide

Gene Aliases: ATPMB, ATPSB

ID gène: 171374

UniProt: P10719

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process