Calmodulin 1 Kit ELISA

N° du produit ABIN5652505

Détail du produit Calmodulin 1 Kit ELISA

Antigène: Calmodulin 1 (Calm1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calmodulin (CAM). No significant cross-reactivity or interference between Calmodulin (CAM) and analogues was observed.

Sensibilité: 0.072 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calmodulin (CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calmodulin (CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calmodulin (CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calmodulin (CAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calmodulin (CAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calmodulin (CAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Calmodulin 1 (Calm1)

Autre désignation: Calmodulin (Calm1 Produits)

Synonymes: AI256814 Kit ELISA, AI327027 Kit ELISA, AI461935 Kit ELISA, AL024000 Kit ELISA, CaM Kit ELISA, Calm Kit ELISA, CaMI Kit ELISA, CALML2 Kit ELISA, CAMI Kit ELISA, CPVT4 Kit ELISA, DD132 Kit ELISA, LQT14 Kit ELISA, PHKD Kit ELISA, caM Kit ELISA, calm1 Kit ELISA, calm2-a Kit ELISA, calm2-b Kit ELISA, calm2a Kit ELISA, calml2 Kit ELISA, cami Kit ELISA, dd132 Kit ELISA, 3909 Kit ELISA, CAM Kit ELISA, CG8472 Kit ELISA, Cal Kit ELISA, Cal49A Kit ELISA, CalA Kit ELISA, Cam1 Kit ELISA, D-CaM Kit ELISA, DmCaM Kit ELISA, DmCalm Kit ELISA, Dmel\CG8472 Kit ELISA, anon-EST:Posey59 Kit ELISA, cam Kit ELISA, dCaM Kit ELISA, l(2)03909 Kit ELISA, l(2)3909 Kit ELISA, lincRNA.S2634 Kit ELISA, calmodulin 1 Kit ELISA, calmodulin 2 Kit ELISA, calmodulin 1 (phosphorylase kinase, delta) L homeolog Kit ELISA, Calmodulin Kit ELISA, Calm1 Kit ELISA, Calm2 Kit ELISA, CALM1 Kit ELISA, calm1.L Kit ELISA, Cam Kit ELISA

Sujet:

Gene Name: Calmodulin

Gene Aliases: CALcium MODULated ProteIN

ID gène: 801

UniProt: P62158

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction, BCR Signaling