CHEK1 Kit ELISA

N° du produit ABIN5652789

Détail du produit CHEK1 Kit ELISA

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Checkpoint Kinase 1 (CHEK1). No significant cross-reactivity or interference between Checkpoint Kinase 1 (CHEK1) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Kinase 1 (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Kinase 1 (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Kinase 1 (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Checkpoint Kinase 1 (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Checkpoint Kinase 1 (CHEK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Checkpoint Kinase 1 (CHEK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: Checkpoint Kinase 1 (CHEK1 Produits)

Synonymes: An08g10320 Kit ELISA, AO090003000441 Kit ELISA, CHK1 Kit ELISA, chk1 Kit ELISA, C85740 Kit ELISA, Chk1 Kit ELISA, rad27 Kit ELISA, id:ibd2720 Kit ELISA, zgc:56093 Kit ELISA, CHEK1 Kit ELISA, checkpoint kinase 1 Kit ELISA, serine/threonine-protein kinase chk1 Kit ELISA, serine/threonine-protein kinase Chk1 Kit ELISA, CAMK/CAMKL/CHK1 protein kinase Chk1 Kit ELISA, checkpoint kinase 1 S homeolog Kit ELISA, CHEK1 Kit ELISA, ANI_1_2436074 Kit ELISA, AOR_1_768154 Kit ELISA, PTRG_04183 Kit ELISA, SJAG_01680 Kit ELISA, PAAG_04978 Kit ELISA, MCYG_03290 Kit ELISA, VDBG_03742 Kit ELISA, chek1.S Kit ELISA, Chek1 Kit ELISA, chek1 Kit ELISA

Sujet:

Gene Name: Checkpoint Kinase 1

Gene Aliases: CHK1, Cell cycle checkpoint kinase, Serine/threonine-protein kinase Chk1

ID gène: 12649

UniProt: O35280

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN