COL2A1 Kit ELISA

N° du produit ABIN5653113

Détail du produit COL2A1 Kit ELISA

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.88 pg/mL - 3000 pg/mL

Seuil minimal de détection: 46.88 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Collagen Type II Alpha 1 (COL2a1). No significant cross-reactivity or interference between Collagen Type II Alpha 1 (COL2a1) and analogues was observed.

Sensibilité: 18.25 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II Alpha 1 (COL2a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type II Alpha 1 (COL2a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II Alpha 1 (COL2a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type II Alpha 1 (COL2a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type II Alpha 1 (COL2a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type II Alpha 1 (COL2a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Autre désignation: Collagen Type II Alpha 1 (COL2A1 Produits)

Synonymes: ANFH Kit ELISA, AOM Kit ELISA, COL11A3 Kit ELISA, SEDC Kit ELISA, Col2 Kit ELISA, Col2a Kit ELISA, Col2a-1 Kit ELISA, Del1 Kit ELISA, Dmm Kit ELISA, Lpk Kit ELISA, M100856 Kit ELISA, Rgsc856 Kit ELISA, CG2A1A Kit ELISA, COLLII Kit ELISA, col2a1a Kit ELISA, CB11 Kit ELISA, col2a1 Kit ELISA, coll2a1 Kit ELISA, fb38c06 Kit ELISA, fc10c01 Kit ELISA, wu:fb38c06 Kit ELISA, wu:fc10c01 Kit ELISA, collagen type II alpha 1 chain Kit ELISA, collagen, type II, alpha 1 Kit ELISA, collagen, type II, alpha 1 L homeolog Kit ELISA, collagen, type II, alpha 1 S homeolog Kit ELISA, collagen, type II, alpha 1a Kit ELISA, COL2A1 Kit ELISA, Col2a1 Kit ELISA, col2a1 Kit ELISA, col2a1.L Kit ELISA, col2a1.S Kit ELISA, col2a1a Kit ELISA

Sujet:

Gene Name: Collagen Type II Alpha 1

Gene Aliases: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

Pathways: Sensory Perception of Sound, Growth Factor Binding