CYP24A1 Kit ELISA

N° du produit ABIN5653499

Détail du produit CYP24A1 Kit ELISA

Antigène: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 24A1 (CYP24A1). No significant cross-reactivity or interference between Cytochrome P450 24A1 (CYP24A1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 24A1 (CYP24A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 24A1 (CYP24A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 24A1 (CYP24A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 24A1 (CYP24A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 24A1 (CYP24A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 24A1 (CYP24A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Autre désignation: Cytochrome P450 24A1 (CYP24A1 Produits)

Synonymes: CP24 Kit ELISA, CYP24 Kit ELISA, HCAI Kit ELISA, P450-CC24 Kit ELISA, 24-OHase Kit ELISA, Cyp24 Kit ELISA, 25-hydroxyvitaminD324-hydroxylase Kit ELISA, VITD12 Kit ELISA, CP450CC24 Kit ELISA, cytochrome P450 family 24 subfamily A member 1 Kit ELISA, cytochrome P450, family 24, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 24, subfamily A, polypeptide 1 Kit ELISA, CYP24A1 Kit ELISA, Cyp24a1 Kit ELISA

Sujet:

Gene Name: Cytochrome P450 24A1

Gene Aliases: CP24, CYP24, P450-CC24, Vitamin D 24-Hydroxylase, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial , Cytochrome P450 Family 24 Subfamily A Polypeptide 1

ID gène: 1591

UniProt: Q07973

Pathways: Metabolism of Steroid Hormones and Vitamin D