DICER1 Kit ELISA

N° du produit ABIN5653660

Détail du produit DICER1 Kit ELISA

Antigène: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dicer 1, Ribonuclease Type III (DICER1). No significant cross-reactivity or interference between Dicer 1, Ribonuclease Type III (DICER1) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dicer 1, Ribonuclease Type III (DICER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dicer 1, Ribonuclease Type III (DICER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dicer 1, Ribonuclease Type III (DICER1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dicer 1, Ribonuclease Type III (DICER1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Autre désignation: Dicer 1, Ribonuclease Type III (DICER1 Produits)

Synonymes: dcr1 Kit ELISA, dicer Kit ELISA, herna Kit ELISA, DICER1 Kit ELISA, dicer1 Kit ELISA, 1110006F08Rik Kit ELISA, D12Ertd7e Kit ELISA, mKIAA0928 Kit ELISA, DCR1 Kit ELISA, Dicer Kit ELISA, HERNA Kit ELISA, MNG1 Kit ELISA, DICER Kit ELISA, fc39d11 Kit ELISA, fc74b05 Kit ELISA, wu:fc39d11 Kit ELISA, wu:fc74b05 Kit ELISA, dicer 1, ribonuclease III Kit ELISA, endoribonuclease Dicer Kit ELISA, dicer 1, ribonuclease III S homeolog Kit ELISA, dicer 1, ribonuclease type III Kit ELISA, dicer 1 ribonuclease III Kit ELISA, DICER1 Kit ELISA, CpipJ_CPIJ010534 Kit ELISA, dicer1 Kit ELISA, dicer1.S Kit ELISA, Dicer1 Kit ELISA

Sujet:

Gene Name: Dicer 1, Ribonuclease Type III

Gene Aliases: Dicer, HERNA, Dicer 1,Double-Stranded RNA-Specific Endoribonuclease, Helicase with RNase motif, Helicase MOI

ID gène: 23405

UniProt: Q9UPY3

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization