Ephrin A5 Kit CLIA

N° du produit ABIN5653910

Détail du produit Ephrin A5 Kit CLIA

Antigène: Ephrin A5 (EFNA5)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ephrin A5 (EFNA5). No significant cross-reactivity or interference between Ephrin A5 (EFNA5) and analogues was observed.

Sensibilité: 6.52 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Ephrin A5 (EFNA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ephrin A5 (EFNA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ephrin A5 (EFNA5) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Ephrin A5 (EFNA5)

Autre désignation: Ephrin A5 (EFNA5 Produits)

Synonymes: EFNA5 Kit CLIA, af1 Kit CLIA, efl5 Kit CLIA, rags Kit CLIA, eplg7 Kit CLIA, lerk7 Kit CLIA, AL-1 Kit CLIA, AV158822 Kit CLIA, EFL-5 Kit CLIA, Ephrin-A5 Kit CLIA, Epl7 Kit CLIA, LERK-7 Kit CLIA, RAGS Kit CLIA, Lerk7 Kit CLIA, AF1 Kit CLIA, EFL5 Kit CLIA, EPLG7 Kit CLIA, GLC1M Kit CLIA, LERK7 Kit CLIA, ephrin A5 Kit CLIA, EFNA5 Kit CLIA, efna5 Kit CLIA, Efna5 Kit CLIA

Sujet:

Gene Name: Ephrin A5

Gene Aliases: AF1, EFL5, EPLG7, GLC1M, LERK7, RAGS, AL-1, EPH-related receptor tyrosine kinase ligand 7

ID gène: 1946

UniProt: P52803

Pathways: Signalisation RTK