ERK2 Kit ELISA

N° du produit ABIN5654029

Détail du produit ERK2 Kit ELISA

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.88 pg/mL - 3000 pg/mL

Seuil minimal de détection: 46.88 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Extracellular Signal Regulated Kinase 2 (ERK2). No significant cross-reactivity or interference between Extracellular Signal Regulated Kinase 2 (ERK2) and analogues was observed.

Sensibilité: 18.29 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 2 (ERK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Signal Regulated Kinase 2 (ERK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Signal Regulated Kinase 2 (ERK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Signal Regulated Kinase 2 (ERK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 2 (ERK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 2 (ERK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Autre désignation: Extracellular Signal Regulated Kinase 2 (MAPK1 Produits)

Synonymes: ERK-1 Kit ELISA, ERK1 Kit ELISA, ERT2 Kit ELISA, HS44KDAP Kit ELISA, HUMKER1A Kit ELISA, P44ERK1 Kit ELISA, P44MAPK Kit ELISA, PRKM3 Kit ELISA, p44-ERK1 Kit ELISA, p44-MAPK Kit ELISA, Erk-1 Kit ELISA, Erk1 Kit ELISA, Ert2 Kit ELISA, Esrk1 Kit ELISA, Mnk1 Kit ELISA, Mtap2k Kit ELISA, Prkm3 Kit ELISA, p44 Kit ELISA, p44erk1 Kit ELISA, p44mapk Kit ELISA, ERK Kit ELISA, ERK2 Kit ELISA, ERT1 Kit ELISA, MAPK2 Kit ELISA, P42MAPK Kit ELISA, PRKM1 Kit ELISA, PRKM2 Kit ELISA, p38 Kit ELISA, p40 Kit ELISA, p41 Kit ELISA, p41mapk Kit ELISA, 9030612K14Rik Kit ELISA, AA407128 Kit ELISA, AU018647 Kit ELISA, C78273 Kit ELISA, Erk2 Kit ELISA, Prkm1 Kit ELISA, p42mapk Kit ELISA, zERK2 Kit ELISA, MAPK1 Kit ELISA, Xp42 Kit ELISA, erk Kit ELISA, erk2 Kit ELISA, ert1 Kit ELISA, mapk Kit ELISA, mapk1b Kit ELISA, mapk2 Kit ELISA, mpk1 Kit ELISA, prkm1 Kit ELISA, prkm2 Kit ELISA, mapk1 Kit ELISA, mapk1a Kit ELISA, xp42 Kit ELISA, ATMPK1 Kit ELISA, F14N23.9 Kit ELISA, F14N23_9 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, MNK1 Kit ELISA, ATMPK2 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 Kit ELISA, T30E16.13 Kit ELISA, T30E16_13 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, mitogen activated protein kinase 1 Kit ELISA, mitogen-activated protein kinase 1 L homeolog Kit ELISA, mitogen-activated protein kinase 1 S homeolog Kit ELISA, mitogen activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, MAPK3 Kit ELISA, Mapk3 Kit ELISA, MAPK1 Kit ELISA, Mapk1 Kit ELISA, mapk1 Kit ELISA, mapk1.L Kit ELISA, mapk1.S Kit ELISA, PfMAP1 Kit ELISA, MPK1 Kit ELISA, PVX_084965 Kit ELISA, LOC9328617 Kit ELISA, Tsp_01601 Kit ELISA, MPK2 Kit ELISA

Sujet:

Gene Name: Extracellular Signal Regulated Kinase 2

Gene Aliases: MAPK1, P38, P40, ERK2, ERT1, MAPK2, P42MAPK, P42-MAPK, PRKM1, PRKM2, P41, P41mapk, P41-mapk, Mitogen-Activated Protein Kinase 1, Mitogen-activated protein kinase 2

ID gène: 5594

UniProt: P28482

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins