Fibrillin 1 Kit CLIA

N° du produit ABIN5654136

Détail du produit Fibrillin 1 Kit CLIA

Antigène: Fibrillin 1 (FBN1)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 20000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fibrillin 1 (FBN1). No significant cross-reactivity or interference between Fibrillin 1 (FBN1) and analogues was observed.

Sensibilité: 10.95 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Fibrillin 1 (FBN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibrillin 1 (FBN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibrillin 1 (FBN1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrillin 1 (FBN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrillin 1 (FBN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Fibrillin 1 (FBN1)

Autre désignation: Fibrillin 1 (FBN1 Produits)

Synonymes: FBN1 Kit CLIA, ACMICD Kit CLIA, ECTOL1 Kit CLIA, FBN Kit CLIA, GPHYSD2 Kit CLIA, MASS Kit CLIA, MFS1 Kit CLIA, OCTD Kit CLIA, SGS Kit CLIA, SSKS Kit CLIA, WMS Kit CLIA, WMS2 Kit CLIA, AI536462 Kit CLIA, B430209H23 Kit CLIA, Fib-1 Kit CLIA, Tsk Kit CLIA, fibrillin 1 Kit CLIA, FBN1 Kit CLIA, Fbn1 Kit CLIA

Sujet:

Gene Name: Fibrillin 1

Gene Aliases: FBN, MASS, MFS1, OCTD, SGS, Asprosin

ID gène: 2200

UniProt: P35555

Pathways: Maintenance of Protein Location, SARS-CoV-2 Protein Interactome