FOXO1 Kit ELISA

N° du produit ABIN5654409

Détail du produit FOXO1 Kit ELISA

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein O1 (FOXO1). No significant cross-reactivity or interference between Forkhead Box Protein O1 (FOXO1) and analogues was observed.

Sensibilité: 0.119 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein O1 (FOXO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein O1 (FOXO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein O1 (FOXO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein O1 (FOXO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein O1 (FOXO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein O1 (FOXO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Autre désignation: Forkhead Box Protein O1 (FOXO1 Produits)

Synonymes: FKH1 Kit ELISA, FKHR Kit ELISA, FOXO1A Kit ELISA, FoxO1a.1 Kit ELISA, foxo1 Kit ELISA, zgc:153388 Kit ELISA, Fkhr Kit ELISA, Foxo1a Kit ELISA, AI876417 Kit ELISA, Afxh Kit ELISA, Fkhr1 Kit ELISA, fkh1 Kit ELISA, fkhr Kit ELISA, foxo1a Kit ELISA, xFoxO1 Kit ELISA, FoxO1A Kit ELISA, foxo1a.2 Kit ELISA, forkhead box O1 Kit ELISA, forkhead box O1 a Kit ELISA, forkhead box O1 L homeolog Kit ELISA, forkhead box O1 b Kit ELISA, FOXO1 Kit ELISA, foxo1a Kit ELISA, Foxo1 Kit ELISA, foxo1 Kit ELISA, foxo1.L Kit ELISA, foxo1b Kit ELISA

Sujet:

Gene Name: Forkhead Box Protein O1

Gene Aliases: FOX-O1, FKH1, FKHR, FOXO1A, Forkhead Homolog In Rhabdomyosarcoma

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling