ITGB1 Kit ELISA

N° du produit ABIN5655500

Détail du produit ITGB1 Kit ELISA

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Integrin Beta 1 (ITGb1). No significant cross-reactivity or interference between Integrin Beta 1 (ITGb1) and analogues was observed.

Sensibilité: 0.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 1 (ITGb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 1 (ITGb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 1 (ITGb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 1 (ITGb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 1 (ITGb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 1 (ITGb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Autre désignation: Integrin Beta 1 (ITGB1 Produits)

Synonymes: CD29 Kit ELISA, FNRB Kit ELISA, GPIIA Kit ELISA, MDF2 Kit ELISA, MSK12 Kit ELISA, VLA-BETA Kit ELISA, VLAB Kit ELISA, 4633401G24Rik Kit ELISA, AA409975 Kit ELISA, AA960159 Kit ELISA, ENSMUSG00000051907 Kit ELISA, Fnrb Kit ELISA, Gm9863 Kit ELISA, gpIIa Kit ELISA, cd29 Kit ELISA, fnrb Kit ELISA, gpiia Kit ELISA, itgb1 Kit ELISA, mdf2 Kit ELISA, msk12 Kit ELISA, vla-beta Kit ELISA, vlab Kit ELISA, itgb1-a Kit ELISA, itgb1-b Kit ELISA, integrin subunit beta 1 Kit ELISA, integrin beta 1 (fibronectin receptor beta) Kit ELISA, integrin subunit beta 1 S homeolog Kit ELISA, integrin beta 1 Kit ELISA, integrin subunit beta 1 L homeolog Kit ELISA, ITGB1 Kit ELISA, Itgb1 Kit ELISA, itgb1.S Kit ELISA, LOC100009173 Kit ELISA, itgb1.L Kit ELISA

Sujet:

Gene Name: Integrin Beta 1

Gene Aliases: CD29, MSK12, ITG-B1, FNRB, GPIIA, MDF2, MSK12, VLAB, Fibronectin Receptor Beta Polypeptide, Antigen CD29 Includes MDF2, Glycoprotein IIa, VLA-4 subunit beta

ID gène: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome