Kazald1 Kit ELISA

N° du produit ABIN5656174

Détail du produit Kazald1 Kit ELISA

Antigène: Kazald1 (Kazal-Type Serine Peptidase Inhibitor Domain 1 (Kazald1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). No significant cross-reactivity or interference between Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Kazald1 (Kazal-Type Serine Peptidase Inhibitor Domain 1 (Kazald1))

Autre désignation: Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (Kazald1 Produits)

Synonymes: MGC80370 Kit ELISA, igfbp-rp10 Kit ELISA, Xigfbp-rp10 Kit ELISA, bono1 Kit ELISA, fksg28 Kit ELISA, fksg40 Kit ELISA, ba108l7.1 Kit ELISA, BONO1 Kit ELISA, FKSG28 Kit ELISA, FKSG40 Kit ELISA, IGFBP-rP10 Kit ELISA, AI842353 Kit ELISA, Bono1 Kit ELISA, Kazal type serine peptidase inhibitor domain 1 Kit ELISA, Kazal type serine peptidase inhibitor domain 1 S homeolog Kit ELISA, Kazal-type serine peptidase inhibitor domain 1 Kit ELISA, KAZALD1 Kit ELISA, kazald1.S Kit ELISA, kazald1 Kit ELISA, Kazald1 Kit ELISA

Sujet:

Gene Name: Kazal Type Serine Protease Inhibitor Domain Containing Protein 1

Gene Aliases: BONO1, FKSG28, FKSG40

ID gène: 81621

UniProt: Q96I82

Pathways: Growth Factor Binding