NR5A2 + LRH1 Kit ELISA
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- Antigène Voir toutes NR5A2 + LRH1 (NR5A2) Kits ELISA
- NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Liver Receptor Homolog 1 (LRH1). No significant cross-reactivity or interference between Liver Receptor Homolog 1 (LRH1) and analogues was observed.
- Sensibilité
- 0.112 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1 (LRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver Receptor Homolog 1 (LRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver Receptor Homolog 1 (LRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver Receptor Homolog 1 (LRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes NR5A2 + LRH1 (NR5A2) Kits ELISA
- NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
- Autre désignation
- Liver Receptor Homolog 1 (NR5A2 Produits)
- Synonymes
- xff1rashort Kit ELISA, NR5A2 Kit ELISA, b1f Kit ELISA, b1f2 Kit ELISA, cpf Kit ELISA, ff1r Kit ELISA, ftf Kit ELISA, ftz-f1 Kit ELISA, ftz-f1beta Kit ELISA, hb1f Kit ELISA, hb1f-2 Kit ELISA, lrh-1 Kit ELISA, lrh1 Kit ELISA, xff1ra Kit ELISA, B1F Kit ELISA, B1F2 Kit ELISA, CPF Kit ELISA, FTF Kit ELISA, FTZ-F1 Kit ELISA, FTZ-F1beta Kit ELISA, LRH-1 Kit ELISA, LRH1 Kit ELISA, hB1F-2 Kit ELISA, xFF1rB Kit ELISA, AU020803 Kit ELISA, D1Ertd308e Kit ELISA, Ftf Kit ELISA, UF2-H3B Kit ELISA, Lrh-1 Kit ELISA, nuclear receptor subfamily 5 group A member 2 S homeolog Kit ELISA, nuclear receptor subfamily 5 group A member 2 Kit ELISA, nuclear receptor subfamily 5 group A member 2 L homeolog Kit ELISA, nuclear receptor subfamily 5, group A, member 2 Kit ELISA, nr5a2.S Kit ELISA, NR5A2 Kit ELISA, nr5a2 Kit ELISA, nr5a2.L Kit ELISA, Nr5a2 Kit ELISA
- Sujet
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Gene Name: Liver Receptor Homolog 1
Gene Aliases: NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, LRH-1, hB1F, hB1F-2, Hepatocytic transcription factor, Alpha-1-fetoprotein transcription factor, Nuclear Receptor Subfamily 5,Group A,Member 2
- ID gène
- 2494
- UniProt
- O00482
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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