MYL2 Kit ELISA
-
- Antigène Voir toutes MYL2 Kits ELISA
- MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 7.8 pg/mL - 500 pg/mL
- Seuil minimal de détection
- 7.8 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 2, Regulatory, Cardiac (MYL2). No significant cross-reactivity or interference between Myosin Light Chain 2, Regulatory, Cardiac (MYL2) and analogues was observed.
- Sensibilité
- 3.1 pg/mL
- Featured
- Discover our best selling MYL2 Kit ELISA
- Top Product
- Discover our top product MYL2 Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 2, Regulatory, Cardiac (MYL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 2, Regulatory, Cardiac (MYL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 2, Regulatory, Cardiac (MYL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 2, Regulatory, Cardiac (MYL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 2, Regulatory, Cardiac (MYL2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 2, Regulatory, Cardiac (MYL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes MYL2 Kits ELISA
- MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))
- Autre désignation
- Myosin Light Chain 2, Regulatory, Cardiac (MYL2 Produits)
- Synonymes
- CMH10 Kit ELISA, MLC2 Kit ELISA, 2410014J02Rik Kit ELISA, MLC-2 Kit ELISA, Mlc2 Kit ELISA, MLC-2v Kit ELISA, Mlc2v Kit ELISA, Mylpc Kit ELISA, Mlc-2 Kit ELISA, myl2 Kit ELISA, zgc:136848 Kit ELISA, XMLC2 Kit ELISA, cmh10 Kit ELISA, mlc2 Kit ELISA, G2 Kit ELISA, MLC-2A Kit ELISA, MLC2V Kit ELISA, myosin light chain 2 Kit ELISA, myosin light chain, phosphorylatable, fast skeletal muscle Kit ELISA, myosin, light polypeptide 2, regulatory, cardiac, slow Kit ELISA, myosin, light chain 2b, regulatory, cardiac, slow Kit ELISA, myosin light chain 2 S homeolog Kit ELISA, myosin, light chain 2, regulatory, cardiac, slow Kit ELISA, MYL2 Kit ELISA, Mylpf Kit ELISA, Myl2 Kit ELISA, myl2b Kit ELISA, myl2.S Kit ELISA
- Sujet
-
Gene Name: Myosin Light Chain 2, Regulatory, Cardiac
Gene Aliases: CMH10, MLC2, Myosin,Light Chain 2,Regulatory,Cardiac,Slow, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
- ID gène
- 4633
- UniProt
- P10916
- Pathways
- Myometrial Relaxation and Contraction
-