NPC2 Kit ELISA
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- Antigène Voir toutes NPC2 Kits ELISA
- NPC2 (Niemann-Pick Disease, Type C2 (NPC2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.781 ng/mL - 50 ng/mL
- Seuil minimal de détection
- 0.781 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Niemann Pick Disease Type C2 (NPC2). No significant cross-reactivity or interference between Niemann Pick Disease Type C2 (NPC2) and analogues was observed.
- Sensibilité
- 0.31 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Niemann Pick Disease Type C2 (NPC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Niemann Pick Disease Type C2 (NPC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Niemann Pick Disease Type C2 (NPC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Niemann Pick Disease Type C2 (NPC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Niemann Pick Disease Type C2 (NPC2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Niemann Pick Disease Type C2 (NPC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes NPC2 Kits ELISA
- NPC2 (Niemann-Pick Disease, Type C2 (NPC2))
- Autre désignation
- Niemann Pick Disease Type C2 (NPC2 Produits)
- Synonymes
- 2700012J19Rik Kit ELISA, AA408070 Kit ELISA, AU045843 Kit ELISA, HE1 Kit ELISA, EDDM1 Kit ELISA, re1 Kit ELISA, CE1 Kit ELISA, EPI-1 Kit ELISA, cb292 Kit ELISA, sb:cb292 Kit ELISA, NPC intracellular cholesterol transporter 2 Kit ELISA, Niemann-Pick disease, type C2 Kit ELISA, Npc2 Kit ELISA, NPC2 Kit ELISA, npc2 Kit ELISA
- Sujet
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Gene Name: Niemann Pick Disease Type C2
Gene Aliases: HE1, NP-C2, EDDM1, Epididymal Protein 1, Human epididymis-specific protein 1, Epididymal secretory protein E1
- ID gène
- 10577
- UniProt
- P61916
- Pathways
- SARS-CoV-2 Protein Interactome
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