SULT1A1 Kit ELISA

N° du produit ABIN5657753

Détail du produit SULT1A1 Kit ELISA

Antigène: SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phenol Sulfotransferase (PST). No significant cross-reactivity or interference between Phenol Sulfotransferase (PST) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phenol Sulfotransferase (PST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phenol Sulfotransferase (PST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phenol Sulfotransferase (PST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phenol Sulfotransferase (PST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phenol Sulfotransferase (PST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phenol Sulfotransferase (PST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))

Autre désignation: Phenol Sulfotransferase (SULT1A1 Produits)

Synonymes: HAST1/HAST2 Kit ELISA, P-PST Kit ELISA, PST Kit ELISA, ST1A1 Kit ELISA, ST1A3 Kit ELISA, STP Kit ELISA, STP1 Kit ELISA, TSPST1 Kit ELISA, AI266890 Kit ELISA, Stp Kit ELISA, Stp1 Kit ELISA, ASTIV Kit ELISA, Mx-ST Kit ELISA, PST-1 Kit ELISA, St1a1 Kit ELISA, Stm Kit ELISA, Sult1a3 Kit ELISA, pst Kit ELISA, st1a3 Kit ELISA, stp Kit ELISA, stp1 Kit ELISA, tspst1 Kit ELISA, cSULT1A1 Kit ELISA, SULT1A2 Kit ELISA, SULT1A1 Kit ELISA, SIAT8-D Kit ELISA, ST8SiaIV Kit ELISA, Siat8d Kit ELISA, PST1 Kit ELISA, SIAT8D Kit ELISA, ST8SIA-IV Kit ELISA, sulfotransferase family 1A member 1 Kit ELISA, sulfotransferase family 1A, phenol-preferring, member 1 Kit ELISA, sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 Kit ELISA, sulfotransferase family 1A member 1 S homeolog Kit ELISA, sulfotransferase 1A1 Kit ELISA, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 Kit ELISA, SULT1A1 Kit ELISA, Sult1a1 Kit ELISA, sult1a1 Kit ELISA, sult1a1.S Kit ELISA, LOC704658 Kit ELISA, LOC709318 Kit ELISA, St8sia4 Kit ELISA, ST8SIA4 Kit ELISA, LOC100064221 Kit ELISA

Sujet:

Gene Name: Phenol Sulfotransferase

Gene Aliases: SULT1A1, HAST1/HAST2, ST1A3, STP1, TSPST1, Sulfotransferase 1A1, Aryl sulfotransferase 1, Phenol sulfotransferase 1, Thermostable phenol sulfotransferase

ID gène: 83783

UniProt: P17988