SERPINB6 Kit ELISA

N° du produit ABIN5657845

Détail du produit SERPINB6 Kit ELISA

Antigène: SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Placental Thrombin Inhibitor (PTI). No significant cross-reactivity or interference between Placental Thrombin Inhibitor (PTI) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placental Thrombin Inhibitor (PTI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placental Thrombin Inhibitor (PTI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placental Thrombin Inhibitor (PTI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placental Thrombin Inhibitor (PTI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placental Thrombin Inhibitor (PTI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placental Thrombin Inhibitor (PTI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))

Autre désignation: Placental Thrombin Inhibitor (SERPINB6 Produits)

Synonymes: Serpinb6a Kit ELISA, cap Kit ELISA, dfnb91 Kit ELISA, mstp057 Kit ELISA, pi6 Kit ELISA, pti Kit ELISA, serpinb8 Kit ELISA, spi3 Kit ELISA, 4930482L21Rik Kit ELISA, D330015H01Rik Kit ELISA, PI-6 Kit ELISA, Serpinb6 Kit ELISA, Spi3 Kit ELISA, ovalbumin Kit ELISA, CAP Kit ELISA, DFNB91 Kit ELISA, MSTP057 Kit ELISA, PI6 Kit ELISA, PTI Kit ELISA, SPI3 Kit ELISA, serpin family B member 6A Kit ELISA, serpin family B member 6 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade B, member 6a Kit ELISA, serpin peptidase inhibitor, clade B (ovalbumin), member 6 Kit ELISA, Serpinb6a Kit ELISA, serpinb6 Kit ELISA, SERPINB6 Kit ELISA

Sujet:

Gene Name: Placental Thrombin Inhibitor

Gene Aliases: SERPINB6, CAP, PI6, SPI3, Serpin Peptidase Inhibitor Clade B Member 6, Cytoplasmic antiproteinase, Peptidase inhibitor 6

ID gène: 5269

UniProt: P35237