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PDK1 Kit ELISA

PDK1 Reactivité: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5658316
  • Antigène Voir toutes PDK1 Kits ELISA
    PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))
    Reactivité
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56 ng/mL - 100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) and analogues was observed.
    Sensibilité
    0.58 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes PDK1 Kits ELISA
    PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))
    Autre désignation
    Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1 Produits)
    Synonymes
    ATP5A Kit ELISA, B830012B01 Kit ELISA, D530020C15Rik Kit ELISA, si:zfos-979f1.3 Kit ELISA, 3-phosphoinositide dependent protein kinase-1 Kit ELISA, pyruvate dehydrogenase kinase 1 Kit ELISA, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) Kit ELISA, pyruvate dehydrogenase kinase, isoenzyme 1 Kit ELISA, pyruvate dehydrogenase kinase, isozyme 1 Kit ELISA, pyruvate dehydrogenase kinase 1 L homeolog Kit ELISA, Pdpk1 Kit ELISA, PDK1 Kit ELISA, ATP5G2 Kit ELISA, Pdk1 Kit ELISA, pdk1 Kit ELISA, pdk1.L Kit ELISA
    Sujet

    Gene Name: Pyruvate Dehydrogenase Kinase Isozyme 1

    Gene Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

    Pathways
    Signalisation PI3K-Akt, Signalisation RTK, Regulation of Carbohydrate Metabolic Process, Hepatitis C, L'effet Warburg
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