VDAC1 Kit ELISA

N° du produit ABIN5659974

Détail du produit VDAC1 Kit ELISA

Antigène: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Voltage Dependent Anion Channel Protein 1 (VDAC1). No significant cross-reactivity or interference between Voltage Dependent Anion Channel Protein 1 (VDAC1) and analogues was observed.

Sensibilité: 0.131 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Voltage Dependent Anion Channel Protein 1 (VDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Voltage Dependent Anion Channel Protein 1 (VDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Autre désignation: Voltage Dependent Anion Channel Protein 1 (VDAC1 Produits)

Synonymes: vdac1 Kit ELISA, VDAC1 Kit ELISA, 5076 Kit ELISA, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1 Kit ELISA, ATVDAC1 Kit ELISA, T22N4.9 Kit ELISA, T22N4_9 Kit ELISA, voltage dependent anion channel 1 Kit ELISA, PORIN Kit ELISA, VDAC-1 Kit ELISA, AL033343 Kit ELISA, Vdac5 Kit ELISA, fa13f11 Kit ELISA, wu:fa13f11 Kit ELISA, zgc:85830 Kit ELISA, voltage-dependent anion channel 1 S homeolog Kit ELISA, voltage-dependent anion channel 1 Kit ELISA, voltage dependent anion channel 1 Kit ELISA, vdac1.S Kit ELISA, vdac1 Kit ELISA, VDAC1 Kit ELISA, Vdac1 Kit ELISA

Sujet:

Gene Name: Voltage Dependent Anion Channel Protein 1

Gene Aliases: PORIN-31-HL, Porin 31HM, Plasmalemmal porin, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1

ID gène: 7416

UniProt: P21796