WNT5B Kit ELISA

N° du produit ABIN5660022

Détail du produit WNT5B Kit ELISA

Antigène: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) and analogues was observed.

Sensibilité: 0.55 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Autre désignation: Wingless Type MMTV Integration Site Family, Member 5B (WNT5B Produits)

Synonymes: wnt-5b Kit ELISA, xwnt5b Kit ELISA, WNT5B Kit ELISA, AW545702 Kit ELISA, Wnt-5b Kit ELISA, xwnt-5c Kit ELISA, CHUNP6928 Kit ELISA, id:ibd5111 Kit ELISA, ppt Kit ELISA, wnt-5 Kit ELISA, wnt5 Kit ELISA, wnt[b] Kit ELISA, wu:fk85g06 Kit ELISA, Wnt family member 5B Kit ELISA, wingless-type MMTV integration site family, member 5B Kit ELISA, Wnt family member 5B S homeolog Kit ELISA, wingless-type MMTV integration site family, member 5b Kit ELISA, wnt5b Kit ELISA, WNT5B Kit ELISA, Wnt5b Kit ELISA, wnt5b.S Kit ELISA

Sujet:

Gene Name: Wingless Type MMTV Integration Site Family, Member 5B

Pathways: Signalisation WNT, Embryonic Body Morphogenesis, Positive Regulation of fat Cell Differentiation