COL2A1 Kit ELISA

N° du produit ABIN5674675

Détail du produit COL2A1 Kit ELISA

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0055 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Autre désignation: CollagenType II Alpha 1 (COL2a1) (COL2A1 Produits)

Synonymes: ANFH Kit ELISA, AOM Kit ELISA, COL11A3 Kit ELISA, SEDC Kit ELISA, Col2 Kit ELISA, Col2a Kit ELISA, Col2a-1 Kit ELISA, Del1 Kit ELISA, Dmm Kit ELISA, Lpk Kit ELISA, M100856 Kit ELISA, Rgsc856 Kit ELISA, CG2A1A Kit ELISA, COLLII Kit ELISA, col2a1a Kit ELISA, CB11 Kit ELISA, col2a1 Kit ELISA, coll2a1 Kit ELISA, fb38c06 Kit ELISA, fc10c01 Kit ELISA, wu:fb38c06 Kit ELISA, wu:fc10c01 Kit ELISA, collagen type II alpha 1 chain Kit ELISA, collagen, type II, alpha 1 Kit ELISA, collagen, type II, alpha 1 L homeolog Kit ELISA, collagen, type II, alpha 1 S homeolog Kit ELISA, collagen, type II, alpha 1a Kit ELISA, COL2A1 Kit ELISA, Col2a1 Kit ELISA, col2a1 Kit ELISA, col2a1.L Kit ELISA, col2a1.S Kit ELISA, col2a1a Kit ELISA

Sujet: Alternative name: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

ID gène: 12824

UniProt: P28481

Pathways: Sensory Perception of Sound, Growth Factor Binding