Aromatase Kit ELISA

N° du produit ABIN5674814

Détail du produit Aromatase Kit ELISA

Antigène: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0117 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Autre désignation: Aromatase (ARO) (CYP19A1 Produits)

Synonymes: ARO Kit ELISA, ARO1 Kit ELISA, CPV1 Kit ELISA, CYAR Kit ELISA, CYP19 Kit ELISA, CYPXIX Kit ELISA, P-450AROM Kit ELISA, Aromatase Kit ELISA, Cyp19 Kit ELISA, Cyp19a Kit ELISA, p450arom Kit ELISA, P450 arom Kit ELISA, aro Kit ELISA, cyp19 Kit ELISA, cyp19a Kit ELISA, p450arom-A Kit ELISA, P450AROM Kit ELISA, Ar Kit ELISA, ArKO Kit ELISA, Int-5 Kit ELISA, Int5 Kit ELISA, CYP19P1 Kit ELISA, CYP19A1 Kit ELISA, CYP19a1 Kit ELISA, P450aromA Kit ELISA, ar1 Kit ELISA, cypXIX Kit ELISA, etID309862.18 Kit ELISA, zgc:136541 Kit ELISA, AroB Kit ELISA, CYP19a2 Kit ELISA, P450aromB Kit ELISA, cyp19b Kit ELISA, zgc:92614 Kit ELISA, cyp19a1 Kit ELISA, cytochrome P450 family 19 subfamily A member 1 Kit ELISA, cytochrome P450, family 19, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450 family 19 subfamily A member 1 L homeolog Kit ELISA, aromatase Kit ELISA, cytochrome P450 aromatase Kit ELISA, cytochrome P450, family 19, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450 19A1 Kit ELISA, cytochrome P450, family 17, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 19 Kit ELISA, cytochrome P450, family 19, subfamily A, polypeptide 1a Kit ELISA, cytochrome P450, family 19, subfamily A, polypeptide 1b Kit ELISA, cytochrome P450 19A1-like Kit ELISA, CYP19A1 Kit ELISA, Cyp19a1 Kit ELISA, cyp19a1.L Kit ELISA, SAV_2383 Kit ELISA, pfcyp19b-tstr Kit ELISA, CYP19 Kit ELISA, cyp19a1a Kit ELISA, cyp19a1b Kit ELISA, LOC101174651 Kit ELISA

Sujet: Alternative name: CYP19A1, CYPXIX, ARO, ARO1, CPV1, CYAR, CYP19, P450AROM, P450-AROM, Estrogen synthase, Cytochrome P450,Family 19,Subfamily A,Polypeptide 1

ID gène: 13075

UniProt: P28649

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis