C-JUN Kit ELISA

N° du produit ABIN5674851

Kit ELISA C-JUN Souris, Colorimetric test pour la quantification de Souris C-JUN.

Aperçu rapide pour C-JUN Kit ELISA (ABIN5674851)

Antigène: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.391 ng/mL - 25 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit C-JUN Kit ELISA

Seuil minimal de détection: 0.391 ng/mL

Analytical Method: Quantitative

Sensibilité: 0172 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Autre désignation: V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)

Sujet: Alternative name: C-Jun, AP1, CJun, p39, Jun Oncogene, Proto-oncogene c-Jun, Activator protein 1, Transcription factor AP-1

ID gène: 16476

UniProt: P05627

Pathways: Signalisation MAPK, Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins