Parkin Kit ELISA

N° du produit ABIN5690927

Détail du produit Parkin Kit ELISA

Antigène: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Parkin ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Parkin.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Autre désignation: Parkin (PARK2 Produits)

Synonymes: CG10523 Kit ELISA, Dmel\\CG10523 Kit ELISA, Dpark Kit ELISA, SD01679 Kit ELISA, dpk Kit ELISA, AR-JP Kit ELISA, LPRS2 Kit ELISA, PDJ Kit ELISA, PRKN Kit ELISA, Park Kit ELISA, Prkn Kit ELISA, si:ch211-123f21.1 Kit ELISA, zgc:112390 Kit ELISA, pdr-1 Kit ELISA, PARK2 Kit ELISA, parkin Kit ELISA, parkin RBR E3 ubiquitin protein ligase Kit ELISA, Parkinson disease (autosomal recessive, juvenile) 2, parkin Kit ELISA, park Kit ELISA, PRKN Kit ELISA, Prkn Kit ELISA, prkn Kit ELISA, CpipJ_CPIJ014867 Kit ELISA, Park2 Kit ELISA

ID gène: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway