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ERK1 Kit ELISA

MAPK3 Reactivité: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5706913
  • Antigène Voir toutes ERK1 (MAPK3) Kits ELISA
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Reactivité
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.625 ng/mL - 40 ng/mL
    Seuil minimal de détection
    0.625 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0.249 ng/mL
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Pre-coated, ready to use 96-well strip plate,
    Standard (freeze dried),
    Standard Diluent,
    Detection Reagent A,
    Detection Reagent B,
    Assay Diluent A,
    Assay Diluent B,
    TMB substrate,
    Stop Solution,
    Wash Buffer(30 x concenthumane),
    Plate sealer for 96 wells,
    Instruction manual

    Volume d'échantillon
    100 µL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes ERK1 (MAPK3) Kits ELISA
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Autre désignation
    Extracellular Signal Regulated Kinase 1 (ERK1) (MAPK3 Produits)
    Synonymes
    ERK-1 Kit ELISA, ERK1 Kit ELISA, ERT2 Kit ELISA, HS44KDAP Kit ELISA, HUMKER1A Kit ELISA, P44ERK1 Kit ELISA, P44MAPK Kit ELISA, PRKM3 Kit ELISA, p44-ERK1 Kit ELISA, p44-MAPK Kit ELISA, Erk-1 Kit ELISA, Erk1 Kit ELISA, Ert2 Kit ELISA, Esrk1 Kit ELISA, Mnk1 Kit ELISA, Mtap2k Kit ELISA, Prkm3 Kit ELISA, p44 Kit ELISA, p44erk1 Kit ELISA, p44mapk Kit ELISA, ERK3 Kit ELISA, ERK6 Kit ELISA, P38GAMMA Kit ELISA, PRKM12 Kit ELISA, SAPK-3 Kit ELISA, SAPK3 Kit ELISA, fi06b09 Kit ELISA, wu:fi06b09 Kit ELISA, zERK1 Kit ELISA, Tb08.10J17.940 Kit ELISA, MAPK1 Kit ELISA, MNK1 Kit ELISA, AW123708 Kit ELISA, Erk6 Kit ELISA, P38gamma Kit ELISA, Prkm12 Kit ELISA, Sapk3 Kit ELISA, ATMAPK3 Kit ELISA, ATMPK3 Kit ELISA, T6D9.4 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 12 Kit ELISA, mitogen activated protein kinase 3 Kit ELISA, mitogen-activated serine/threonine-protein kinase Kit ELISA, MAPK3 Kit ELISA, Mapk3 Kit ELISA, MAPK12 Kit ELISA, mapk3 Kit ELISA, Tc00.1047053509475.10 Kit ELISA, Tb927.8.3550 Kit ELISA, Mapk12 Kit ELISA, CEK1 Kit ELISA, MPK3 Kit ELISA
    Sujet
    Alternative name: HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
    Pathways
    Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
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