TNFRSF10A Kit ELISA

N° du produit ABIN625358

Détail du produit TNFRSF10A Kit ELISA

Antigène: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 40-10.000 pg/mL

Seuil minimal de détection: 40 pg/mL

Application: ELISA

Fonction: Custom Human TRAIL Receptor 1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes human TRAIL R1.

Sensibilité: 40 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Autre désignation: TRAIL R1 (TNFRSF10A Produits)

Synonymes: APO2 Kit ELISA, CD261 Kit ELISA, DR4 Kit ELISA, TRAILR-1 Kit ELISA, TRAILR1 Kit ELISA, TNFRSF10A Kit ELISA, TNF receptor superfamily member 10a Kit ELISA, tumor necrosis factor receptor superfamily member 26 Kit ELISA, tumor necrosis factor receptor superfamily, member 10a Kit ELISA, TNFRSF10A Kit ELISA, LOC510798 Kit ELISA

Sujet: Gene Names: TNFSF10 APO2L TRAIL
Protein names: Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)

ID gène: 8797

UniProt: O00220

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity