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EGFR Kit IQ-ELISA

EGFR Reactivité: Rat qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN6384487
  • Antigène Voir toutes EGFR IQ-ELISA Kits
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Reactivité
    • 35
    • 25
    • 22
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Rat
    Méthode de détection
    qPCR
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat Immunoquantitative (PCR-Based) EGFR ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Semi-Quantitative
    Attributs du produit
    IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
    Ingrédients
    • EGFR Microplate (Item A): 96 well PCR plate coated with anti-Rat EGFR
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Rat EGFR
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for EGFR (Item F): 2 vials of a 4x concentrated solution of anti-Rat EGFR affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Matériel non inclus
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
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  • Indications d'application
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Commentaires

    The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

    Volume d'échantillon
    25 µL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes EGFR IQ-ELISA Kits
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Autre désignation
    EGFR (EGFR Produits)
    Synonymes
    C-erb Kit IQ-ELISA, CG10079 Kit IQ-ELISA, D-EGFR Kit IQ-ELISA, D-Egf Kit IQ-ELISA, DEGFR Kit IQ-ELISA, DER Kit IQ-ELISA, DER flb Kit IQ-ELISA, DER/EGFR Kit IQ-ELISA, DER/faint little ball Kit IQ-ELISA, DER/top Kit IQ-ELISA, DER/torpedo Kit IQ-ELISA, DER1 Kit IQ-ELISA, DEgfr Kit IQ-ELISA, Degfr Kit IQ-ELISA, Der Kit IQ-ELISA, DmHD-33 Kit IQ-ELISA, Dmel\\CG10079 Kit IQ-ELISA, EFG-R Kit IQ-ELISA, EGF-R Kit IQ-ELISA, EGFR Kit IQ-ELISA, EGFr Kit IQ-ELISA, EGfr Kit IQ-ELISA, EK2-6 Kit IQ-ELISA, Egf Kit IQ-ELISA, Egf-r Kit IQ-ELISA, EgfR Kit IQ-ELISA, El Kit IQ-ELISA, Elp Kit IQ-ELISA, Elp-1 Kit IQ-ELISA, Elp-B1 Kit IQ-ELISA, Elp-B1RB1 Kit IQ-ELISA, HD-33 Kit IQ-ELISA, TOP Kit IQ-ELISA, Torpedo/DER Kit IQ-ELISA, Torpedo/Egfr Kit IQ-ELISA, c-erbB Kit IQ-ELISA, d-egf-r Kit IQ-ELISA, dEGFR Kit IQ-ELISA, dEGFR1 Kit IQ-ELISA, dEgfr Kit IQ-ELISA, der Kit IQ-ELISA, egfr Kit IQ-ELISA, flb Kit IQ-ELISA, l(2)05351 Kit IQ-ELISA, l(2)09261 Kit IQ-ELISA, l(2)57DEFa Kit IQ-ELISA, l(2)57EFa Kit IQ-ELISA, l(2)57Ea Kit IQ-ELISA, mor1 Kit IQ-ELISA, top Kit IQ-ELISA, top/DER Kit IQ-ELISA, top/flb Kit IQ-ELISA, torpedo/Egfr Kit IQ-ELISA, torpedo/egfr Kit IQ-ELISA, EGFR12 Kit IQ-ELISA, EGFR15 Kit IQ-ELISA, egfr1 Kit IQ-ELISA, Erbb2 Kit IQ-ELISA, ERBB Kit IQ-ELISA, ERBB1 Kit IQ-ELISA, HER1 Kit IQ-ELISA, PIG61 Kit IQ-ELISA, mENA Kit IQ-ELISA, ErbB-1 Kit IQ-ELISA, Errp Kit IQ-ELISA, 9030024J15Rik Kit IQ-ELISA, AI552599 Kit IQ-ELISA, Erbb Kit IQ-ELISA, Errb1 Kit IQ-ELISA, Wa5 Kit IQ-ELISA, wa-2 Kit IQ-ELISA, wa2 Kit IQ-ELISA, epidermal growth factor receptor Kit IQ-ELISA, Epidermal growth factor receptor Kit IQ-ELISA, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Kit IQ-ELISA, EGFR Kit IQ-ELISA, Egfr Kit IQ-ELISA, egfra Kit IQ-ELISA, egfr1 Kit IQ-ELISA, LOC5564544 Kit IQ-ELISA
    UniProt
    G3V6K6
    Pathways
    Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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