E-cadherin Kit ELISA

N° du produit ABIN6719781

Détail du produit E-cadherin Kit ELISA

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Arc 1, Cadherin 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD 234, CD324, CD324 antigen, CDH1, CDHE, ECAD, Epithelial cadherin, epithelial calcium dependant adhesion protein, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin

Background: E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.

Gene Name: CDH1

Production: Natural and recombinant mouse E-Cadherin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D157-V709

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse E-Cadherin standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: E-Cadherin (CDH1 Produits)

Synonymes: Arc-1 Kit ELISA, CD324 Kit ELISA, CDHE Kit ELISA, ECAD Kit ELISA, LCAM Kit ELISA, UVO Kit ELISA, E-cadherin Kit ELISA, cdh1 Kit ELISA, si:dz180o5.2 Kit ELISA, uvo Kit ELISA, lcam Kit ELISA, E-Cad Kit ELISA, XBcad Kit ELISA, l-cam Kit ELISA, xcdh1 Kit ELISA, cdhc-A Kit ELISA, xb-cad Kit ELISA, XTCAD-1 Kit ELISA, c-cadherin Kit ELISA, cadherin-1 Kit ELISA, uvomorulin Kit ELISA, XB-cadherin Kit ELISA, arc-1 Kit ELISA, cd324 Kit ELISA, cdhe Kit ELISA, ecad Kit ELISA, AA960649 Kit ELISA, E-cad Kit ELISA, Ecad Kit ELISA, L-CAM Kit ELISA, Um Kit ELISA, cadherin 1 Kit ELISA, si:busm1-180o5.2 Kit ELISA, cadherin 1, type 1, E-cadherin (epithelial) Kit ELISA, cadherin 1, type 1 S homeolog Kit ELISA, CDH1 Kit ELISA, si:busm1-180o5.2 Kit ELISA, cdh1 Kit ELISA, Cdh1 Kit ELISA, cdh1.S Kit ELISA

ID gène: 12550

NCBI Accession: NP_033994

UniProt: P09803

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation