TWEAK Kit ELISA

N° du produit ABIN6719961

Détail du produit TWEAK Kit ELISA

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: APO 3 ligand, APO 3L, APO3 ligand, APO3/DR3 ligand, APO3L, CD255, DR3LG, MGC129581, MGC20669, TNF related weak inducer of apoptosis, TNF-related weak inducer of apoptosis, TNF12_HUMAN, TNFRS12a, TNFSF 12, TNFSF12, TNFSF12 protein, Tumor necrosis factor (ligand) superfamily member 12, Tumor necrosis factor ligand superfamily member 12, Tumor necrosis factor superfamily member 12, TWEAK, UNQ181/PRO207

Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis(TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.

Gene Name: TNFSF12

Production: Natural and recombinant mouse TNFSF12. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: S94-H249

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFSF12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.. It is recommended that each mouse TNFSF12 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Autre désignation: TNFSF12 - TWEAK (TNFSF12 Produits)

Synonymes: APO3L Kit ELISA, DR3LG Kit ELISA, TWEAK Kit ELISA, Apo3l Kit ELISA, Dr3l Kit ELISA, Dr3lg Kit ELISA, Tweak Kit ELISA, TNF superfamily member 12 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 12 Kit ELISA, TNFSF12 Kit ELISA, Tnfsf12 Kit ELISA

ID gène: 21944

NCBI Accession: NP_035744

UniProt: O54907

Pathways: Apoptose