SERPINA4 Kit ELISA

N° du produit ABIN6720111

Détail du produit SERPINA4 Kit ELISA

Antigène: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: KAIN_HUMAN, Kallikrein inhibitor, Kallistatin, Peptidase inhibitor 4, PI-4, PI4, Serpin A4, SERPINA4

Background: Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Gene Name: SERPINA4

Production: Natural and recombinant human Kallistatin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q21-P427

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Kallistatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Kallistatin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Autre désignation: Kallistatin - Serpina4 (SERPINA4 Produits)

Synonymes: KLKR Kit ELISA, Klk6 Kit ELISA, hK1 Kit ELISA, KAL Kit ELISA, KLST Kit ELISA, KST Kit ELISA, PI-4 Kit ELISA, PI4 Kit ELISA, kallistatin Kit ELISA, SERPINA4 Kit ELISA, DKFZp469E1737 Kit ELISA, KALps Kit ELISA, Klst Kit ELISA, kallikrein 1 Kit ELISA, serpin family A member 4 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 Kit ELISA, KLK1 Kit ELISA, SERPINA4 Kit ELISA, Serpina4 Kit ELISA, SPIA4 Kit ELISA, Serpina4-ps1 Kit ELISA

ID gène: 3815

NCBI Accession: NP_000213

UniProt: P10721