IFNB1 Kit ELISA (Interferon, beta 1, Fibroblast)

Details for Product IFNB1 Kit ELISA No. ABIN6730353
Antigène
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
7
6
5
5
5
5
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4
4
3
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3
1
1
1
1
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.34 ng/mL - 75 ng/mL
Seuil minimal de détection
0.34 ng/mL
Application
ELISA
Options
Fonction Mouse IFN-beta ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This ELISA antibody pair recognizes Mouse IFN-beta.
Attributs du produit
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Antigène
Autre désignation IFN-beta (IFNB1 ELISA Kit Extrait)
UniProt P01575
Pathways Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy
Indications d'application Optimal working dilution should be determined by the investigator.
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Préparation des réactifs

Recommended Dilution for serum and plasma samples2 fold

Restrictions For Research Use only
Stockage commentaire The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption 6 months