CSNK2A1/CK II alpha Kit ELISA

N° du produit ABIN6730466

Détail du produit CSNK2A1/CK II alpha Kit ELISA

Antigène: CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse CSNK2A1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse CSNK2A1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))

Autre désignation: CSNK2A1 (CSNK2A1 Produits)

Synonymes: CK2A2 Kit ELISA, CSNK2A1 Kit ELISA, CK2A1 Kit ELISA, CKII Kit ELISA, CSNK2A3 Kit ELISA, CK2A Kit ELISA, Ck2a Kit ELISA, BmCK2a Kit ELISA, wu:fi38e04 Kit ELISA, wu:fi38h03 Kit ELISA, csnk2a1 Kit ELISA, CK-II Kit ELISA, CK2 Kit ELISA, Ckiialpha Kit ELISA, Csnk2a1-rs4 Kit ELISA, ck2a1 Kit ELISA, casein kinase 2 alpha 2 Kit ELISA, casein kinase 2 alpha 1 Kit ELISA, casein kinase 2 alpha subunit Kit ELISA, casein kinase 2, alpha 1 polypeptide Kit ELISA, CK2 protein kinase alpha 2 Kit ELISA, casein kinase II alpha subunit Kit ELISA, Casein kinase II alpha subunit Kit ELISA, casein kinase 2, alpha 1 polypeptide S homeolog Kit ELISA, CSNK2A2 Kit ELISA, CSNK2A1 Kit ELISA, ck2a Kit ELISA, Ck2a Kit ELISA, csnk2a1 Kit ELISA, cka2 Kit ELISA, Ckiialpha Kit ELISA, CK2A1 Kit ELISA, Csnk2a1 Kit ELISA, csnk2a1.S Kit ELISA

UniProt: Q60737

Pathways: SARS-CoV-2 Protein Interactome