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ERK2 Kit ELISA
MAPK1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730481
Détail du produit ERK2 Kit ELISA
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Antigène
Voir toutes ERK2 (MAPK1) Kits ELISA
ERK2 (MAPK1)
(Mitogen-Activated Protein Kinase 1 (MAPK1))
Reactivité
Toutes les réactivités sur ERK2 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human MAPK1/ERK2 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human MAPK1.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes ERK2 (MAPK1) Kits ELISA
ERK2 (MAPK1)
(Mitogen-Activated Protein Kinase 1 (MAPK1))
Autre désignation
MAPK1 (ERK2 (MAPK1 Produits )
Synonymes
ERK-1 Kit ELISA, ERK1 Kit ELISA, ERT2 Kit ELISA, HS44KDAP Kit ELISA, HUMKER1A Kit ELISA, P44ERK1 Kit ELISA, P44MAPK Kit ELISA, PRKM3 Kit ELISA, p44-ERK1 Kit ELISA, p44-MAPK Kit ELISA, Erk-1 Kit ELISA, Erk1 Kit ELISA, Ert2 Kit ELISA, Esrk1 Kit ELISA, Mnk1 Kit ELISA, Mtap2k Kit ELISA, Prkm3 Kit ELISA, p44 Kit ELISA, p44erk1 Kit ELISA, p44mapk Kit ELISA, ERK Kit ELISA, ERK2 Kit ELISA, ERT1 Kit ELISA, MAPK2 Kit ELISA, P42MAPK Kit ELISA, PRKM1 Kit ELISA, PRKM2 Kit ELISA, p38 Kit ELISA, p40 Kit ELISA, p41 Kit ELISA, p41mapk Kit ELISA, 9030612K14Rik Kit ELISA, AA407128 Kit ELISA, AU018647 Kit ELISA, C78273 Kit ELISA, Erk2 Kit ELISA, Prkm1 Kit ELISA, p42mapk Kit ELISA, zERK2 Kit ELISA, MAPK1 Kit ELISA, Xp42 Kit ELISA, erk Kit ELISA, erk2 Kit ELISA, ert1 Kit ELISA, mapk Kit ELISA, mapk1b Kit ELISA, mapk2 Kit ELISA, mpk1 Kit ELISA, prkm1 Kit ELISA, prkm2 Kit ELISA, mapk1 Kit ELISA, mapk1a Kit ELISA, xp42 Kit ELISA, ATMPK1 Kit ELISA, F14N23.9 Kit ELISA, F14N23_9 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, MNK1 Kit ELISA, ATMPK2 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 Kit ELISA, T30E16.13 Kit ELISA, T30E16_13 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, mitogen activated protein kinase 1 Kit ELISA, mitogen-activated protein kinase 1 L homeolog Kit ELISA, mitogen-activated protein kinase 1 S homeolog Kit ELISA, mitogen activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, MAPK3 Kit ELISA, Mapk3 Kit ELISA, MAPK1 Kit ELISA, Mapk1 Kit ELISA, mapk1 Kit ELISA, mapk1.L Kit ELISA, mapk1.S Kit ELISA, PfMAP1 Kit ELISA, MPK1 Kit ELISA, PVX_084965 Kit ELISA, LOC9328617 Kit ELISA, Tsp_01601 Kit ELISA, MPK2 Kit ELISA
UniProt
P28482
Pathways
Signalisation MAPK , Signalisation RTK , Apoptose , Interferon-gamma Pathway , Fc-epsilon Receptor Signaling Pathway , Response to Growth Hormone Stimulus , Activation of Innate immune Response , Cellular Response to Molecule of Bacterial Origin , Hepatitis C , Protein targeting to Nucleus , Toll-Like Receptors Cascades , Monocarboxylic Acid Catabolic Process , Autophagy , G-protein mediated Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Signaling of Hepatocyte Growth Factor Receptor , VEGFR1 Specific Signals , BCR Signaling , S100 Proteins
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