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WNT10B Kit ELISA
WNT10B
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730526
Détail du produit WNT10B Kit ELISA
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Antigène
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WNT10B
(Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))
Reactivité
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Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse Wnt-10b ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse Wnt-10b.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes WNT10B Kits ELISA
WNT10B
(Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))
Autre désignation
Wnt-10b (WNT10B Produits )
Synonymes
WNT10B Kit ELISA, Xwnt-10b Kit ELISA, Xwnt10b Kit ELISA, wnt-12 Kit ELISA, Wnt12 Kit ELISA, SHFM6 Kit ELISA, WNT-12 Kit ELISA, Xwnt-10 Kit ELISA, wnt10 Kit ELISA, wnt10-A Kit ELISA, Zwnt[d] Kit ELISA, wnt10br Kit ELISA, wnt[d] Kit ELISA, Wnt family member 10B Kit ELISA, wingless-type MMTV integration site family, member 10B Kit ELISA, Wnt family member 10B S homeolog Kit ELISA, wingless-type MMTV integration site family, member 10b Kit ELISA, WNT10B Kit ELISA, wnt10b Kit ELISA, Wnt10b Kit ELISA, wnt10b.S Kit ELISA
UniProt
P48614
Pathways
Signalisation WNT , Skeletal Muscle Fiber Development
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