ACTH Kit ELISA

N° du produit ABIN6730845

Détail du produit ACTH Kit ELISA

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivité: Souris, Humain, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human/Mouse/Rat Adrenocorticotropic Hormone EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat ACTH

Sensibilité: 0.5 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: Adrenocorticotropic hormone (ACTH Produits)

Synonymes: ACTH Kit ELISA, BE Kit ELISA, Beta-LPH Kit ELISA, Clip Kit ELISA, Gamma-LPH Kit ELISA, Npp Kit ELISA, Pomc-1 Kit ELISA, Pomc1 Kit ELISA, alpha-MSH Kit ELISA, alphaMSH Kit ELISA, beta-MSH Kit ELISA, gamma-MSH Kit ELISA, CLIP Kit ELISA, LPH Kit ELISA, MSH Kit ELISA, NPP Kit ELISA, POC Kit ELISA, pro-opiomelanocortin-alpha Kit ELISA, proopiomelanocortin Kit ELISA, Pomc Kit ELISA, POMC Kit ELISA

Classe de substances: Hormone

ID gène: 5443

UniProt: P01194

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity